We likewise demonstrate striking differences in regulation of membrane trafficking of NBCe 1 vs. In contrast, BLM NBCn 1 was not downregulated in parotid acinar cells treated with constitutive recycling inhibitors, cholinergic stimulators, or PMA. The PKC inhibitor, GF-109203X, blocked this redistribution, indicating a role for PKC. Carbachol and PMA likewise caused redistribution of NBCe 1 from BLM to early endosomes. Inhibition of constitutive recycling with the carboxylic ionophore monensin or the calmodulin antagonist W-13 caused NBCe 1 to accumulate in early endosomes with a parallel loss from the BLM, suggesting that NBCe 1 is constitutively endocytosed. NBCe 1 and NBCn 1 colocalized with E-cadherin monoclonal antibody at the basolateral membrane (BLM) in polarized ParC5 cells. Here, we studied cholinergic regulation of NBCe 1 and NBCn 1 membrane trafficking by confocal fluorescent microscopy and surface biotinylation in parotid epithelial cells.
We previously reported that angiotensin regulates NBCe 1 by endocytosis in Xenopus oocytes. Electrogenic and electroneutral Na(+)-HCO(3)(-) cotransporters (NBCe 1 and NBCn 1) are abundant in parotid glands. Saliva bicarbonate is essential for maintaining oral health. Perry, Clint Quissell, David O Reyland, Mary E Grichtchenko, Irina IĬholinergic agonists are major stimuli for fluid secretion in parotid acinar cells. Electrogenic NBCe 1 ( SLC 4 A 4), but not electroneutral NBCn 1 ( SLC 4 A 7), cotransporter undergoes cholinergic-stimulated endocytosis in salivary ParC5 cells.
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